Facts-enriched characterization of mobile populations. Morphological data from visuals can add for the richness of flow cytometry knowledge. Such as, the figure displays an or else traditional apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Every single inhabitants to expose morphologically unique characteristics. These insights couldn't are obtained from multiplex staining by itself.
As samples are obtained about the Attune CytPix Flow Cytometer, the higher-pace brightfield digicam captures and stores photographs of detected functions at a fee up to six,000 images for every 2nd, according to the flow fee and impression size. For larger flexibility, Attune Cytometric Software program permits you to alter the impression seize frequency as desired and select a selected gate for picture seize, if demanded.
Combine acoustic concentrating technologies with high-parameter analysis for adaptable spectral unmixing and conventional workflows.
Uncover new insights into your experimental info by visualizing populations employing merged fluorescence and morphology facts. Our software program interface wizards help downsampling, concatenation (merged files) and dimensionality instruments which can be built in the software for an intuitive, streamlined info Examination encounter.
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Listed here, a skilled user has gated singlets confidently. After analyzing the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate has a lot more than 4% aggregates.
Optimizing gating strategy. Even sturdy guide singlet gating is error-susceptible and continues to be a subjective selection place in Nearly all flow cytometry assays. Imaging can be used to substantiate and adjust gates to include only single cells of curiosity.
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The Attune NxT cytometer is the trustworthy workhorse instrument your lab can rely on for dependable and reliable effectiveness. The Attune CytPix model incorporates a substantial-speed brightfield digicam and AI-pushed impression Examination computer software, with recent updates like device Mastering resources and an intuitive consumer interface, enabling Increased info precision, precision, and more quickly insights into sample biology.
People can watch party pictures in serious-time to confirm sample quality, enhance protocols, and record the morphology of each and every cell population determined for Assessment. Imaging seize also Positive aspects from acoustic concentrating, which really helps to place the cells to ensure a sharp, centered impression is acquired.
Audio waves help specific mobile alignment, leading to Remarkable knowledge good quality and ~10X a lot quicker sample flow rates.
We Formerly shown the strength of imaging Car or truck-T/Ramos mobile interactions. Enable’s check out just the population of finest curiosity, the double good occasions, To find out more. We could now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to further more examine the features of such populations and refine gating on these occasions, expanding facts robustness.
Leverage the strength of State-of-the-art engineering and innovation to speed up your discoveries. Belief during the Attune Flow Cytometer loved ones to deliver the trustworthiness, pace, and precision that established your work apart.
No clean/no lyse leukocyte Examination with imaging. Cells ended up obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Expert UX/UI permits end users to prepare AI versions for expanded purposes. (A) The “Practice Model” attribute provides annotation https://genteel-llama-zwq5nb.mystrikingly.com/blog/flow-cytometer-a-revolutionary-tool-for-cell-analysis applications and actual-time design teaching accuracy.
At the center of every Attune Flow Cytometer lies our revolutionary acoustic focusing know-how, which utilizes sound waves to meticulously align cells within the flow cell. This ground breaking strategy surpasses regular hydrodynamic techniques, leading to faster processing speeds, Extraordinary data top quality, and increased sample throughput.
The Attune Xenith cytometer can be a significant-parameter spectral analyzer, providing both spectral unmixing and traditional flow capabilities for detailed and flexible cell Investigation.
Agilent Benefit Assure guarantees you at least a decade of instrument use from your date you purchase. Normally, We're going to credit score you While using the residual price of the procedure towards an upgraded model.
Here, we demonstrate that by gating strategies utilizing image-based quantitative parameters can distinguish interacting cells from coincident functions to far more properly evaluate interacting cells.